Tb green® premix ex taqtm ii kit
WebOct 7, 2024 · The target gene and β-actin expression levels were detected using the TB Green ® Premix Ex TaqTM II Kit (TaKaRa, Tokyo, Japan). The reaction was performed … WebTB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration …
Tb green® premix ex taqtm ii kit
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WebBacterial cells were harvested after induction for 20, 40, 60 and 90 min. Total RNA was extracted and reverse transcribed to cDNA, and real-time PCR was performed using TB Green TM Premix Ex TaqTM II kit (TaKaRa) on the CFX Connect (Bio-Rad) with primers listed in Supplementary Table S3. WebSep 4, 2024 · TaKaRa TB Green ® Premix Ex TaqTM II kit (TAKARA Bio, Japan) was chosen for qRT-PCR, and the instrument used was QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Singapore). Every sample was tested in three biological replicates and three technical replicates, which showed good consistency.
WebMar 1, 2024 · TB Green ® Premix Ex Taq ™ II (Tli RNaseH Plus) 40 Rxns ￥525: Takara RR820A TB Green ® Premix Ex Taq ™ II (Tli RNaseH Plus) 200 Rxns ￥1,785 ￥893: … WebDec 21, 2024 · RNA of L. cubeba was extracted and reverse transcribed by the method provided by Zhao et al. (2024). qRT-PCR was carried out with the assistance of an ABI PRISM 7500 instrument and the TB Green ® Premix Ex TaqTM II kit.
Web36673306 bids are invited for takara chemicals mse emeraldamp gt pcr master mix , primescript tm first strand cdna synthesis kit , agarose routine 00g , tb green premix ex taqtm ii tli rnaseh plus , one kb dna ladder dye plus total quantity : 5 WebJul 22, 2024 · RNA was extracted from equal volume of the five combined fractions using TRIzol reagents (Life Technologies, Shanghai, China), reversely transcribed to cDNA using the PrimeScriptTM RT reagent Kit (Takara, Beijing, China) and amplified by qTR-PCR analysis using the TB Green Premix Ex TaqTM II kit (Takara, Beijing, China).
WebMay 21, 2024 · Total RNAs were isolated from cells using the TRIzol reagent and reverse-transcribed into cDNAs using PrimeScript TM RT reagent Kit. Quantitative PCR (qPCR) …
WebTB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. … taxes online free ontariotaxes online in canadaWebJan 20, 2024 · RNA was reverse-transcribed into cDNA using a PrimeScript ™ RT reagent kit with a gDNA Eraser kit (Takara, Dalian, China), and qRT-PCR was performed with a TB Green Premix Ex TaqTM II kit (Takara, Dalian, China). Three technical replicates were performed for each target gene. the chiddingstone castle pubWebTB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration … taxes online free filing canadaWebJul 16, 2024 · Using GAPDH as an internal reference gene, RT-qPCR was performed using TB Green TM Premix Ex TaqTM II (Takara). The reaction system was as follows: TB Green Premix Ex Taq II (Tli RNaseH Plus) 10 μL, PCR forward primer (10 μM) 0.8 μL, PCR reverse primer (10 μM) 0.8 μL, DNA (< 100 ng) 2 μL, and sterilized water 6.4 μL. taxes online ontarioWebFeb 3, 2024 · Reverse transcription of total RNA was performed following Prime Script ™ RT reagent Kit with gDNA Eraser (Takara, China), TB Green ® Premix Ex TaqTM II(TAKARA, Beijing, China) instructions. The reverse transcription and qPCR of miRNA were performed with miRcute Plus miRNA First-Strand cDNA Kit and miRNA Plus miRNA qPCR Kit … the chief advantage of shipping by truck isWebAfter denatured template DNA at 95°C for 10 s, 40 cycles of 95°C for 5 s, and 60°C for 20 s were performed using TB Green ® Premix Ex TaqTM II Kit (TAKARA), 21 on the ABI StepOne/StepOnePlus Real-Time System. the chi disney plus