Taq dna polymerase 5 u/μl
WebAccurate Taq enzyme 是一种性能优越的热稳定性 DNA 聚合酶，是将Thermus aquaticus DNA Polymerase gene 克隆表达得到。使用本品得到的 PCR 产物 3’ 端带有一个 A 碱 … WebCloned Pfu DNA polymerase (2.5 U/μl) 100 Ua 500 Ub 1000 Uc 5000 Ud ... For Taq DNA polymerase-based PCR amplifications, an extension time of 0.5-1.0 minute/kb of template amplified is usually sufficient for maximum synthesis of a PCR target. In contrast, Pfu DNA polymerase-based PCR amplifications require a
Taq dna polymerase 5 u/μl
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WebTaq DNA Polymerase is used in a variety of techniques: • The enzyme activity is stable during prolonged incubation at high temperatures (+95°C) and can therefore be used to … WebFastStart ™ Taq DNA Polymerase, 5 U/μl. dNTPs included: no, hotstart, suitable for PCR. View Price and Availability. Sigma-Aldrich. 71086. KOD Hot Start DNA Polymerase. High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.
WebDescription. Storage: −20°C. UNSPSC Code: 12352200. Components: FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl; PCR Reaction Buffer, with 20 mM MgCl 2 10x concentrated; PCR Reaction Buffer, without MgCl 2 10x concentrated; MgCl 2 Stock Solution 25 mM; GC-RICH Solution 5x concentrated. RIDADR: NONH for all modes of ... WebPlatinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a …
Web反应体系 （50 μl） 组分名称 反应终浓度 加入量 Accurate Taq DNA Polymerase （5U/μl） 1.25 U 0.25 μl 10X Taq PCR Buffer (Mg2+ plus) 1 x 5 μl dNTP Mix (10mM each) 0.2 mM 1 μl Template < 500 ng - Primer F 0.2 – 1.0 μM - Primer R 0.2 – 1.0 μM - RNase free water — Up to 50 μl 实验操作 Accurate Biotechnology(Hunan ... WebVolume activity: ≥5 U/μL Unit definition: One unit Taq DNA Polymerase is defined as the amount of heat-activated enzyme that incorporates 10 nmol of total …
Web23 giu 2024 · Taq DNA polymerase, 5 U/μL, 10× Taq buffer and MgSO 4 (BioBasic, Canada). 7. DNA template: Gene of interest cloned into the vector pVAX1. In this example, the 3 kbp gene expresses a trans-splicing-based suicide RNA. 8. Q5 ® high-fidelity DNA polymerase, 2000 U/mL and 5× Q5 Reaction Buffer (New England BioLabs, USA). 9. …
WebFastStart ™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting Protocol PCR OPTIMIZATION The choice of the PCR enzyme in combination with an appropriate … food truck bennwihr gareWebDNA Template b Taq HS DNA polymerase (5 U/μl)c To 50 μl 5 μl 1 μl (0.2 mM each) 0.1 - 1 μM 0.1 - 1 μM x μl 1 μl a. b. For most PCR, the optimal final concentration of Mg 2+ is … food truck bazaar fashion square mallWebSource: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/μl Purity: 95% homogeneous by SDS-PAGE Endonuclease: None detected Amplification effiency: Functional PCR Storage: –20°C. ... •100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase •1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl ... food truck bahia blancaWebThermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of … electric outboard for tenderWebPlatinum® Taq DNA Polymerase High Fidelity is supplied at the same 5 unit per μl concentration as Platinum® Taq DNA Polymerase. ... (>15 kb), we recommend using … food truck auto insuranceWebThermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR … food truck awning windowWebCatalog number: 10966018. Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced … food truck basso cambo toulouse