WebNov 6, 2024 · The Lineweaver-Burk Plot. Although it's possible to read K M from a Michaelis-Menten plot, it isn't easy or necessarily accurate. An alternative is to plot the reciprocal of the Michaelis-Menten equation, which is (after all terms have been rearranged): ... How to Calculate Vmax Lineweaver. WebSep 1, 2024 · - GraphPad Prism 9 Curve Fitting Guide Tthe Lineweaver–Burk plot (or two reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934 (Figure \(\PageIndex{2}\)).
GraphPad Prism 9 Curve Fitting Guide - Equation: Michaelis-Menten mo…
WebMar 28, 2024 · 配制3个不同浓度的探针底物溶液，在孵育体系中，分别加入不同浓度的探针药物和芫花醇提物反应一定时间后，终止反应，按“2.4.1”项下处理样品， HPLC分析代谢产物（E-3-G）的含量，根据Lineweaver-Burk和Dixon-Plot图，计算抑制常数Ki，判断抑制类型。 2.7 数据处理 WebJul 30, 2024 · The values of the kinetic parameters (K m and V max) were calculated from the Lineweaver–Burk plots by using GraphPad Prism, version 8.0.1 (GraphPad Software Inc., San Diego, CA, USA), and the inhibition constant (K i) was determined as the intersection on the x-axis of the plot of 1/V max versus different concentrations of … danbury aquarium society
Substrate-Velocity Curves and Lineweaver-Burk Plots1
WebGraphPad WebThe Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation , in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant. Taking reciprocals of both sides of this equation it becomes as follows: WebGraphPad Software Inc lineweaver burk double reciprocal plots Lineweaver Burk Double Reciprocal Plots, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more danbury apple store