Dna ligands for aflatoxin and zearalenone
WebSep 15, 2024 · Aptamers are single strand oligonucleotides selected in vitro by Systematic Evolution of Ligands by Exponential ... Simultaneous detection of aflatoxin B1, ochratoxin A, zearalenone and deoxynivalenol in corn and wheat using surface plasmon resonance ... G.A. Penner, DNA ligands for aflatoxin and zearalenone, US patent … WebAug 20, 2010 · The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and …
Dna ligands for aflatoxin and zearalenone
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WebOct 24, 2024 · Notably, St MCO could directly degrade aflatoxin B 1 (AFB 1) and zearalenone (ZEN) in the absence of mediators. Meanwhile, the presence of various lignin unit-derived natural mediators or ABTS could significantly accelerate the degradation of AFB 1 and ZEN by St MCO. WebSep 1, 2024 · Aflatoxin B 1 and zearalenone were simultaneously determined with high selectivity. Abstract Mycotoxins contamination in agricultural products poses a serious threat to human and animal health, so rapid and sensitive nanosensors for simultaneous determination of multiple mycotoxins in food samples are highly desirable for food safety …
WebA method for the identification of DNA ligands that bind to Zea characterized in that the method comprises: (a) contacting a random library of single stranded DNA sequences to immobilized Zea under... WebPreviously, we designed five new aptamers to aflatoxin B 1 (AFB1) based on a known aptamer sequence (Patent: PCT/CA2010/001 292, Apt1) through a genetic algorithm-based in silico maturation strategy and experimentally measured their affinity to the target toxin. Here, integrated molecular dynamic simulation (MDs) studies with molecular ...
WebThe present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and zearalenone … WebAfter 8 rounds of SELEX (systematic evolution of ligands by exponential enrichment) with an affinity-based monitor and counter-screening process, the ssDNA aptamer Z100 was obtained, which had high affinity (dissociation constant = …
WebDNA LIGANDS FOR AFLATOXIN AND ZEARALENONE United States Application US20120245494 Note: If you have problems viewing the PDF, please make sure you have the latest version of Adobe Acrobat . Back to full text
WebJun 15, 2014 · Ochratoxin A (OTA), zearalenone (ZEN), α-zearalenol, and aflatoxin M1 (AFM 1) were purchased from Sigma-Aldrich (USA). Aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2), and fumonisin (FB1) were purchased from Pribolab Co. Ltd (Singapore). Streptavidin was obtained from Sangon Biotechnology Co. Ltd. (Shanghai, … how to create an msds binderWebNov 9, 2024 · Notably, S.DNAzyme cross-reacted with three other tested aflatoxins, including aflatoxin B2, aflatoxin G1 and aflatoxin M1, revealing the potential for a broadly applicable method to identify the family of aflatoxins. how to create an ms teams sitemicrosoft print pdf windows 10Webzearalenone aflatoxin dna ligands ligands dna Prior art date 2009-08-21 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Withdrawn Application number EP10809412.9A Other languages English (en) French (fr) how to create an ncaa eligibility accountWebDec 1, 2016 · Aflatoxins are naturally occurring mycotoxins that contaminate food and agro commodities, leading to acute and chronic health conditions in human and animals. In the present work, an attempt was made to generate high-affinity single stranded DNA aptamers that specifically bind to Aflatoxin B1 (AFB1) by a modified Systemic Evolution of … how to create an mp4 slideshowWebMay 11, 2012 · DNA2链的NaCl浓度为300mmol/L，此时的孵化率为30.6%。 本实验中加入SDS的主要作用一是增加纳米金的稳定性，降低其对管壁的吸附性；二是缩短了实验时间，以前的孵化一般都要在48h以上，本实验只需10多个小时；三是在维持纳米金颜色不变的同时增加了NaCl的浓度，进而增加了纳米金表面DNA的附着量，这对于以纳米金和DNA链 … how to create an ms teams channelWebThe present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and zearalenone … microsoft print screen command