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Purification of a synthetic oligonucleotide by anion exchange ... - PubMed
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Jagjit Kailey - Scientist 1 (Oligonucleotide …
WebÄKTA oligosynt™ Compact, fully automated system for small-scale synthesis and process development Just launched! OligoPilot™ Robust system designed to produce … WebFeb 24, 2024 · Adding salt to the mRNA preparation suppresses the repulsion charges, enabling the oligo dT to capture the mRNA molecules while impurities are washed away. … WebSep 30, 2005 · A single-step chromatographic method for purification of a synthetic 20-mer oligonucleotide is described. Method optimisation was conducted at laboratory scale where 30 mg crude sample was purified per run with a yield of 17 mg pure oligonucleotide. The protocol was scaled-up in steps to achieve 5-, 58- and a final 230-fold scale-up. how to treat itchy balls